Composite

Part:BBa_K3766191:Design

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-10-21)


LacI K325R expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1431
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

directed evolution from LacI K325R BBa_K3766190

References